Influence of Light Conditions and Medium Composition on Morphophysiological Characteristics of Stevia rebaudiana Bertoni In Vitro and In Vivo

We investigated the influence of different conditions (light composition and plant growth regulators (PGRs) in culture media) on morphophysiological parameters Stevia rebaudiana Bertoni vitro vivo. Both PGRs light spectra applied were found to significantly affect morphogenesis. During micropropagation stage S. rebaudiana, optimal growth, with a multiplication coefficient 15, was obtained an MS medium containing 2,4-epibrassinolide (Epin) indole-3-acetic acid (IAA) at concentrations 0.1 0.5 mg L?1, respectively. rooting stage, we that addition L?1 hydroxycinnamic (Zircon) led root formation frequency 85% resulted strong plants well-developed leaf blades. Cultivation media Epin IAA receiving coherent irradiation weekly basis 100% increase coefficient, better adventitious shoot 33% number leaves. microshoots, cultured 1.0 6-benzylaminopurine (BAP) red monochrome treatments, increased by 30% compared controls (white light, without PGRs).